E952 (Sodium cyclamate) is a chemical compound, the sodium salt of cyclamic acid produced by chemical synthesis with cyclohexane and sulphamic acid as raw materials, and comes in the form of a white crystalline powder or crystals, odourless with an intensely sweet taste.

What it is used for and where

Food

Ingredient included in the list of European food additives as  E952

Used as a non-nutritive sweetener with a sweetening capacity of 30 to 50 times more than sugar in a wide variety of food products: Bakery products, Desserts, Cakes, Jellies, Confectionery, Biscuits, Compound condiments, Table sweeteners, Fruit preserves, Jams and marmalades, Chewing gum, Pickled vegetables, Preserved fruit, etc. It is labelled with the number E959 in the list of European food additives.

This study investigated the effects of the commonly used dietary sweetener sodium cyclamate on osteoblast proliferation and differentiation.  The results indicate that a very low concentration (0.06 μM) of sodium cyclamate can curl and fold microfilament and microtubule of osteoblasts. The addition of sodium cyclamate resulted in a significant decrease in cell viability. Alizarin red staining experiment revealed that sodium cyclamate decreased the mineralization capacity of osteoblasts. The present results suggest that sodium cyclamate can seriously inhibit osteoblast proliferation and differentiation (1).

In this study results indicate that sodium cyclamate can not only bind to the minor groove of DNA, but also intercalate in DNA base pairs.  The binding value for DNA saturation estimated to be 1.67 based on RLS data also indicated that sodium cyclamate may cause DNA damage (2).

Sodium cyclamate other studies

Typical commercial product characteristics Sodium Cyclamate

Appearance	White crystalline powder or crystals
Melting Point	~300 °C   265°C
pH	5.5 - 7.5
Absorbance (270nm)	<0.1
Loss on drying	<10%
Cyclohexylamine	<10 ppm
Dicyclohexylamine	<1 ppm
Sulfate	<500 ppm
Heavy metal	<10ppm
Arsenic	3ppm
Selenium	<30 ppm
Sulfamic Acid	<0.1%
Safety

• Molecular formula  C₆H₁₁NHSO₃.Na      C₆H₁₂NNaO₃S   C₆H₁₂O₃NSNa
• Molecular Weight  201.22 g/mol
• Exact Mass 201.043564
• CAS: 139-05-9
• UNII   1I6F42RME1
• EC Number: 205-348-9
• IUPAC  sodium;N-cyclohexylsulfamate
• InChI=1S/C6H13NO3S.Na/c8-11(9,10)7-6-4-2-1-3-5-6;/h6-7H,1-5H2,(H,8,9,10);/q;+1/p-1  
• InChl Key  UDIPTWFVPPPURJ-UHFFFAOYSA-M    
• SMILES C1CCC(CC1)NS(=O)(=O)[O-].[Na+]
• DSSTox Substance ID: DTXSID6020355
• MDL number  MFCD00003827
• PubChem Substance ID   24871485
• Beilstein/REAXYS Number:  4166868

Synonims: 

• Natriumcyclohexylamidosulfat
• Sodium N-cyclohexylsulphamate
• sodium cyclohexylsulfamate
• Sodium N-Cyclohexylsulfamate
• Cyclohexanesulfamic acid sodium salt

References__________________________________________________________________

(1) Chen Z, Chen G, Zhou K, Zhang P, Ren X, Mei X. Toxicity of food sweetener-sodium cyclamate on osteoblasts cells. Biochem Biophys Res Commun. 2019 Jan 8;508(2):507-511. doi: 10.1016/j.bbrc.2018.11.172. 

(2) Hu Y, Xie M, Wu X. Interaction studies of sodium cyclamate with DNA revealed by spectroscopy methods. Spectrochim Acta A Mol Biomol Spectrosc. 2019 Sep 5;220:117085. doi: 10.1016/j.saa.2019.04.077.