r-Clostridium Histolyticum Collagenase sh-Oligopeptide-60 is a single-chain recombinant fusion peptide, produced by fermentation in E. coli. The starting gene is synthesized by fusing the gene from r-Clostridium Histolyticum Collagenase with sh-Oligopeptide-60. The fused peptide contains 936 amino acids and consists of the proper sequence of the 20 standard amino acids.

Chemical Composition and Structure

The chemical composition of r-Clostridium Histolyticum Collagenase sh-Oligopeptide-60 includes a sequence of 936 amino acids, which are the building blocks of proteins. This sequence is a combination of the amino acid sequences from r-Clostridium Histolyticum Collagenase and sh-Oligopeptide-60, designed to maintain the specific functions of both components.

Physical Properties

r-Clostridium Histolyticum Collagenase sh-Oligopeptide-60 typically appears as a clear to slightly opalescent liquid or as a fine powder when lyophilized. It is soluble in water and appropriate buffers, making it easily incorporable into various formulations.

Production Process

The production of r-Clostridium Histolyticum Collagenase sh-Oligopeptide-60 involves several steps:

Gene Synthesis: The starting gene is synthesized by fusing the gene from r-Clostridium Histolyticum Collagenase with sh-Oligopeptide-60.
Fermentation: The synthesized gene is introduced into E. coli, which is then fermented under controlled conditions to produce the recombinant fusion peptide.
Extraction: The peptide is extracted from the E. coli cells using appropriate methods.
Purification: The extracted peptide undergoes purification processes to remove any impurities and ensure high purity.
Lyophilization (if producing a powder): The purified peptide is lyophilized to produce a fine powder.
Packaging: The final product is packaged under controlled conditions to ensure stability and purity.

Applications

Cosmetics: In the cosmetics industry, r-Clostridium Histolyticum Collagenase sh-Oligopeptide-60 is valued for its potential anti-aging and skin-rejuvenating properties. It is commonly included in formulations of creams, serums, and lotions to improve skin texture and elasticity.
Medical and Pharmaceutical: This fusion peptide is also used in various medical and pharmaceutical applications for its enzymatic activity and potential therapeutic benefits.
Environmental and Safety Considerations

Environmental Impact: The production of r-Clostridium Histolyticum Collagenase sh-Oligopeptide-60 generally has a moderate environmental impact. Sustainable production practices and the use of eco-friendly methods can help minimize the overall impact.
Safety: r-Clostridium Histolyticum Collagenase sh-Oligopeptide-60 is considered safe for topical use in the concentrations typically employed in consumer products. However, it is essential to ensure that the peptide is free from contaminants and complies with regulatory standards. Allergic reactions are rare but cannot be excluded.

Restricted cosmetic ingredient as II/416 a Relevant Item in the Annexes of the European Cosmetics Regulation 1223/2009.

INCI


Skin conditioning agent. It is the mainstay of topical skin treatment as it has the function of restoring, increasing or improving skin tolerance to external factors, including melanocyte tolerance. The most important function of the conditioning agent is to prevent skin dehydration, but the subject is rather complex and involves emollients and humectants that can be added in the formulation.
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