Compendium of the most significant studies with reference to properties, effects.

Pietrocola G, Ceci M, Preda F, Poggio C, Colombo M. Evaluation of the antibacterial activity of a new ozonized olive oil against oral and periodontal pathogens. J Clin Exp Dent. 2018 Nov 1;10(11):e1103-e1108. doi: 10.4317/jced.54929.
Coclusions: The new ozonated oil was a relatively moderate antiseptic. Gram-negative bacteria proved to be more sensitive to ozonized olive oil than Gram-positive ones. ...

Uysal B. Ozonated olive oils and the troubles. J Intercult Ethnopharmacol. 2014 Apr-Jun;3(2):49-50. doi: 10.5455/jice.20140314090801. Abstract: One of the commonly used methods for ozone therapy is ozonated oils. Most prominent type of used oils is extra virgin olive oil. But still, each type of unsaturated oils may be used for ozonation. There are a lot of wrong knowledge on the internet about ozonated oils and its use as well. Just like other ozone therapy studies, also the studies about ozone oils are inadequate to avoid incorrect knowledge. Current data about ozone oil and its benefits are produced by supplier who oversees financial interests and make misinformation. Despite the rapidly increasing ozone oil sales through the internet, its quality and efficacy is still controversial. Dozens of companies and web sites may be easily found to buy ozonated oil. But, very few of these products are reliable, and contain sufficiently ozonated oil. This article aimed to introduce the troubles about ozonated oils and so to inform ozonated oil users.

Colombo M, Ceci M, Felisa E, Poggio C, Pietrocola G. Cytotoxicity evaluation of a new ozonized olive oil. Eur J Dent. 2018 Oct-Dec;12(4):585-589. doi: 10.4103/ejd.ejd_422_18. Conclusions: The present data demonstrate that due to its cytocompatibility, the new ozonized olive oil could be considered an alternative antibacterial agent.

Kumar T, Arora N, Puri G, Aravinda K, Dixit A, Jatti D. Efficacy of ozonized olive oil in the management of oral lesions and conditions: A clinical trial. Contemp Clin Dent. 2016 Jan-Mar;7(1):51-4. doi: 10.4103/0976-237X.177097. Conclusion: Ozone therapy though requires a gaseous form to be more effective, but topical form can also bring out the positive results without any toxicity or side effect. Hence, it can be considered as a minimally invasive therapy for the oral infective and immunological conditions.

Abu-Gharbieh E, Bayoumi FA, Ahmed NG. Alleviation of antioxidant defense system by ozonized olive oil in DNBS-induced colitis in rats. Mediators Inflamm. 2014;2014:967205. doi: 10.1155/2014/967205.

Boland-Nazar NS, Eslamirad Z, Sarmadian H, Ghasemikhah R. An In Vitro Evaluation of Ozonized Organic Extra-Virgin Olive Oil on Giardia Lamblia Cysts. Jundishapur J Microbiol. 2016 Sep 18;9(11):e40839. doi: 10.5812/jjm.40839. Conclusions: We concluded that ozonized organic extra-virgin olive oil was a growth inhibitor of Giardia cysts, and concerning its compatibility with a biological system, it is recommended for further clinical trials.

Ozonated olive oil is the result of a long series of applications of ozone that date back at least fifteen years in dentistry. Ozone (O₃) is a gas that has the property to oxidize inorganic and organic compounds with immediate reaction and is produced by ultraviolet radiation and lightning.

In medicine, ozone can be applied to tissues as ozonated water, gaseous mixture of ozone and oxygen, ozonated water in oil form such as ozonated olive oil. The following are used for oil ozonation: sunflower oil, peanut oil, olive oil, extra virgin olive oil.

Ozonated oil is used in medicine for chronic wound care and in dentistry as an antibacterial to control oral infectious microorganisms in dental plaque and as a subgingival irrigant.

The results of this study demonstrate that application of ozonated olive oil, the topical form of O₃, can accelerate acute skin wound repair in the guinea pig model by promoting collagen synthesis and fibroblast proliferation at the site of injury and increasing the expression of growth factors such as platelet-derived growth factor, growth factor β, and vascular endothelial growth factor. Taken together, we can infer that the application of ozonated olive oil can be considered as an alternative therapeutic modality to improve skin wound healing (1).

This study showed the antibacterial and anti-biofilm activity against S. Mutans of two formulations with ozonated olive oil: Ialozon Blue and Ialozon Pink The study showed the safety of these mouthwashes for daily use, although further data on their efficacy are needed (2).

Ozonated olive oil studies

References_________________________________________________________________

(1) Kim HS, Noh SU, Han YW, Kim KM, Kang H, Kim HO, Park YM. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. J Korean Med Sci. 2009 Jun;24(3):368-74. doi: 10.3346/jkms.2009.24.3.368. 

(2) Nardi GM, Fais S, Casu C, Mazur M, Di Giorgio R, Grassi R, Grassi FR, Orrù G. Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study. Int J Environ Res Public Health. 2020 Dec 6;17(23):9106. doi: 10.3390/ijerph17239106.