Buddleja davidii meristem cell culture extract is derived from the culture of meristematic cells of the Buddleja davidii plant, which belongs to the Scrophulariaceae family. This extract is known for its potent antioxidant, anti-inflammatory, and regenerative properties and is utilized in cosmetics and skincare formulations.

Chemical Composition and Structure

Buddleja davidii meristem cell culture extract contains various bioactive compounds, including:

• Flavonoids: Rich in flavonoids such as quercetin and luteolin, which are recognized for their strong antioxidant and anti-inflammatory effects.
• Phenolic acids: Includes phenolic acids that provide antioxidant protection and contribute to the extract's skin benefits.
• Saponins: Contains saponins that may offer soothing and skin-conditioning properties.
• Vitamins: Provides vitamins like vitamin C, which supports skin health and helps combat oxidative stress.

The chemical structure of Buddleja davidii meristem cell culture extract is characterized by a range of polyphenolic and phytochemical compounds, contributing to its antioxidant and anti-inflammatory properties.

Physical Properties

Buddleja davidii meristem cell culture extract is typically a dark-colored liquid with a vegetal scent. It is soluble in water and aqueous solutions, allowing easy incorporation into various cosmetic formulations.

Production Process 

The production process of Buddleja davidii meristem cell culture extract involves the following steps:

• Cell Culture Initiation: Meristematic cells from Buddleja davidii are cultured in a controlled laboratory environment. These cells are taken from the plant's growing tips and are cultured on specialized media that promotes cell growth and proliferation.
• Preparation: The cultured cells are harvested once they reach the desired density. The cells are then prepared for extraction by processing them into a suitable form.
• Solvent Extraction: The cultured cells are treated with solvents such as alcohol or water to extract the bioactive compounds. This results in a concentrated extract.
• Ultrasound or CO₂ Supercritical Extraction: Advanced methods like ultrasound-assisted extraction or supercritical CO₂ extraction may be employed to enhance the efficiency and quality of the extract.
• Purification: The extract undergoes purification to remove any impurities and to obtain a high-quality product. This process typically includes filtration and concentration steps.
• Formulation: The purified extract is incorporated into cosmetic formulations such as creams, lotions, serums, and masks. It is blended with other ingredients to maximize its skin benefits.
• Quality Control: The final product is subjected to rigorous quality control testing to ensure it meets safety, efficacy, and performance standards. This includes checks on purity, stability, and compatibility with other ingredients.
• Storage: The extract is stored in airtight containers, protected from light, moisture, and heat, to preserve its stability and effectiveness.

Applications

Medical: Buddleja davidii meristem cell culture extract may be used in pharmaceutical formulations for its antioxidant and anti-inflammatory properties (1). It can be applied in treatments for inflammatory conditions and to support overall skin health.

Cosmetic: In cosmetics, Buddleja davidii meristem cell culture extract is valued for its regenerative, soothing, and antioxidant properties. It is used in various products, including creams, lotions, serums, and masks, to improve skin health and protect against environmental stressors.

Other: The extract may also be included in other personal care products and potentially in additional therapeutic applications.

Cosmetics - INCI Functions

• Skin conditioning agent. It is the mainstay of topical skin treatment as it has the function of restoring, increasing or improving skin tolerance to external factors, including melanocyte tolerance. The most important function of the conditioning agent is to prevent skin dehydration, but the subject is rather complex and involves emollients and humectants that can be added in the formulation.

CAS   94465-67-5

EC number   305-351-6

Environmental and Safety Considerations

Buddleja davidii meristem cell culture extract is generally considered safe for use in cosmetics and pharmaceuticals when used as directed. It is a natural ingredient with a low risk of irritation or allergic reactions. Environmentally, the cultivation of meristematic cells in a laboratory setting is more sustainable compared to wild harvesting, as it reduces the ecological impact and supports more sustainable production practices.

References__________________________________________________________________________

(1) Ahmad I, Ahmad N, Wang F. Antioxidant phenylpropanoid glycosides from Buddleja davidii. J Enzyme Inhib Med Chem. 2009 Aug;24(4):993-7. doi: 10.1080/14756360802565072. PMID: 19548780.

Abstract. Phytochemical investigations on the n-BuOH-soluble fraction of the whole plant of Buddleja davidii led to the isolation of the phenylpropanoid glycosides 1-10. Their structures were determined by 1D and 2D NMR spectroscopic techniques. All the compounds showed potent antioxidative activity in three different tests, with IC(50) values in the range 4.15-9.47 microM in the hydroxyl radical ( OH) inhibitory activity test, 40.32-81.15 microM in the total ROS (reactive oxygen species) inhibitory activity test, and 2.26-7.79 microM in the peroxynitrite (ONOO(-)) scavenging activity test. Calceolarioside A (1) displayed the strongest scavenging potential with IC(50) values of (4.15 +/- 0.07, 40.32 +/- 0.09, 2.26 +/- 0.03 microM) for OH, total ROS and scavenging of ONOO(-), respectively.

Wu J, Yi W, Jin L, Hu D, Song B. Antiproliferative and cell apoptosis-inducing activities of compounds from Buddleja davidii in Mgc-803 cells. Cell Div. 2012 Aug 31;7(1):20. doi: 10.1186/1747-1028-7-20. 

Abstract. Background: Buddleja davidii is widely distributed in the southwestern region of China. We have undertaken a systematic analysis of B. davidii as a Chinese traditional medicine with anticancer activity by isolating natural products for their activity against the human gastric cancer cell line Mgc-803 and the human breast cancer cell line Bcap-37. Results: Ten compounds were extracted and isolated from B. davidii, among which colchicine was identified in B. davidii for the first time. The inhibitory activities of these compounds were investigated in Mgc-803, Bcap-37 cells in vitro by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, and the results showed that luteolin and colchicine had potent inhibitory activities against the growth of Mgc-803 cells. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in Mgc-803 cells. The results also showed that the percentages of early apoptotic cells (Annexin V+/PI-, where PI is propidium iodide) and late apoptotic cells (Annexin V+/PI+) increased in a dose- and time-dependent manner. After 36 h of incubation with luteolin at 20 μM, the percentages of cells were approximately 15.4% in early apoptosis and 43.7% in late apoptosis; after 36 h of incubation with colchicine at 20 μM, the corresponding values were 7.7% and 35.2%, respectively. Conclusions: Colchicine and luteolin from B. davidii have potential applications as adjuvant therapies for treating human carcinoma cells. These compounds could also induce apoptosis in tumor cells.

Houghton PJ, Mensah AY, Iessa N, Hong LY. Terpenoids in Buddleja: relevance to chemosystematics, chemical ecology and biological activity. Phytochemistry. 2003 Sep;64(2):385-93. doi: 10.1016/s0031-9422(03)00264-4. 

Abstract. The terpenoids reported from Buddleja species are described. The antifungal activity of chloroform extracts of B. cordata and B. davidii stembark against the soil fungi Fusarium culmorum and Sordari fimicola is reported, with buddledin A shown to be the major compound responsible. The terpenoids present support the view that the Buddlejaceae should be classified in a taxon with Scrophulariaceae rather than Loganiaceae. Ecological aspects of the terpenoids are considered in relation to insects and soil fungi and the role of terpenoids in the chemical basis of the use of Buddleja in traditional medicine is also discussed, especially with regard to their anti-inflammatory properties.

Zhang W, Tang SA, Duan HQ. Studies on chemical constituents of rhizome of Buddleia davidii Zhong Yao Cai. 2009 Apr;32(4):515-7. PMID: 19645234.

Abstract. Objective: To study the chemical constituents of Rhizome of Buddleia davidii. Methods: The chemical constituents were isolated by repeated column chromatography (Toyopearl HW-40C and HPLC) and their structures were elucidated on the basis of physico-chemical properties and spectroscopic features. Results: From the petroleum ether extract of the material, eight compounds were isolated. Their structures were identified as: Buddlindeterpene B(1), Buddledin B(2), Buddledin C(3), deacetyldihydrobuddledin A(4), dihydrobuddledin C(5), Suberosol B(6), Gadain (7) and Hinokinin (8). Conclusion: Compounds 1,4 - 8 are isolated from B. davidii for the first time.