Buddleja davidii leaf cell culture extract is derived from the cultivation of leaf cells of the Buddleja davidii plant, which is a member of the Scrophulariaceae family. This extract is known for its antioxidant, anti-inflammatory, and regenerative properties and is utilized in cosmetic and skincare products.

Chemical Composition and Structure

Buddleja davidii leaf cell culture extract contains several bioactive compounds, including:

• Flavonoids: Rich in flavonoids such as quercetin and luteolin, recognized for their strong antioxidant and anti-inflammatory properties.
• Phenolic acids: Contains phenolic acids that contribute to its antioxidant protection and support skin health.
• Saponins: Includes saponins that may offer soothing and conditioning effects on the skin.
• Vitamins: Provides vitamins like vitamin C, which is beneficial for skin health and protection against oxidative stress.

The chemical structure of Buddleja davidii leaf cell culture extract features a variety of polyphenolic and phytochemical compounds, contributing to its antioxidant and anti-inflammatory benefits.

Physical Properties

Buddleja davidii leaf cell culture extract is typically a dark-colored liquid with a vegetal scent. It is soluble in water and aqueous solutions, allowing it to be easily incorporated into various cosmetic formulations.

Production Process 

The production process of Buddleja davidii leaf cell culture extract involves several steps:

• Cell Culture Initiation: Leaf cells from Buddleja davidii are cultured in a controlled laboratory environment. The cells are grown on specialized media that promotes their growth and proliferation.
• Preparation: The leaf cells are harvested once they reach the desired density. The cells are then prepared for extraction by processing them into a suitable form.
• Solvent Extraction: The leaf cells are treated with solvents such as alcohol or water to extract the bioactive compounds. This process produces a concentrated extract.
• Ultrasound or CO₂ Supercritical Extraction: Advanced methods like ultrasound-assisted extraction or supercritical CO₂ extraction may be employed to enhance the efficiency and quality of the extract.
• Purification: The extract is purified to remove impurities and obtain a high-quality product. This usually involves filtration and concentration processes.
• Formulation: The purified extract is incorporated into various cosmetic formulations, including creams, lotions, serums, and masks. It is combined with other ingredients to maximize its benefits for the skin.
• Quality Control: The final product undergoes rigorous quality control testing to ensure it meets safety, efficacy, and performance standards. This includes checks on purity, stability, and compatibility with other ingredients.
• Storage: The extract is stored in airtight containers, protected from light, moisture, and heat, to maintain its stability and effectiveness.

Applications

Medical: Buddleja davidii leaf cell culture extract may be used in pharmaceutical formulations for its antioxidant and anti-inflammatory properties (1). It can be employed in treatments for inflammatory conditions and to support skin health.

Cosmetic: In cosmetics, Buddleja davidii leaf cell culture extract is valued for its soothing, antioxidant, and conditioning effects. It is used in products such as creams, lotions, serums, and masks to enhance skin health and protect against environmental stressors.

Other: The extract may also be included in other personal care products and potentially in additional therapeutic applications.

Cosmetics - INCI Functions

• Skin conditioning agent. It is the mainstay of topical skin treatment as it has the function of restoring, increasing or improving skin tolerance to external factors, including melanocyte tolerance. The most important function of the conditioning agent is to prevent skin dehydration, but the subject is rather complex and involves emollients and humectants that can be added in the formulation.

CAS   94465-67-5

EC number   305-351-6

Environmental and Safety Considerations

Buddleja davidii leaf cell culture extract is generally considered safe for use in cosmetics and pharmaceuticals when used according to recommended guidelines. It is a natural ingredient with a low risk of irritation or allergic reactions. Environmentally, the laboratory cultivation of leaf cells is a more sustainable approach compared to wild harvesting, reducing ecological impact and supporting sustainable production practices.

References__________________________________________________________________________

(1) Ahmad I, Ahmad N, Wang F. Antioxidant phenylpropanoid glycosides from Buddleja davidii. J Enzyme Inhib Med Chem. 2009 Aug;24(4):993-7. doi: 10.1080/14756360802565072. PMID: 19548780.

Abstract. Phytochemical investigations on the n-BuOH-soluble fraction of the whole plant of Buddleja davidii led to the isolation of the phenylpropanoid glycosides 1-10. Their structures were determined by 1D and 2D NMR spectroscopic techniques. All the compounds showed potent antioxidative activity in three different tests, with IC(50) values in the range 4.15-9.47 microM in the hydroxyl radical ( OH) inhibitory activity test, 40.32-81.15 microM in the total ROS (reactive oxygen species) inhibitory activity test, and 2.26-7.79 microM in the peroxynitrite (ONOO(-)) scavenging activity test. Calceolarioside A (1) displayed the strongest scavenging potential with IC(50) values of (4.15 +/- 0.07, 40.32 +/- 0.09, 2.26 +/- 0.03 microM) for OH, total ROS and scavenging of ONOO(-), respectively.

Wu J, Yi W, Jin L, Hu D, Song B. Antiproliferative and cell apoptosis-inducing activities of compounds from Buddleja davidii in Mgc-803 cells. Cell Div. 2012 Aug 31;7(1):20. doi: 10.1186/1747-1028-7-20. 

Abstract. Background: Buddleja davidii is widely distributed in the southwestern region of China. We have undertaken a systematic analysis of B. davidii as a Chinese traditional medicine with anticancer activity by isolating natural products for their activity against the human gastric cancer cell line Mgc-803 and the human breast cancer cell line Bcap-37. Results: Ten compounds were extracted and isolated from B. davidii, among which colchicine was identified in B. davidii for the first time. The inhibitory activities of these compounds were investigated in Mgc-803, Bcap-37 cells in vitro by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, and the results showed that luteolin and colchicine had potent inhibitory activities against the growth of Mgc-803 cells. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in Mgc-803 cells. The results also showed that the percentages of early apoptotic cells (Annexin V+/PI-, where PI is propidium iodide) and late apoptotic cells (Annexin V+/PI+) increased in a dose- and time-dependent manner. After 36 h of incubation with luteolin at 20 μM, the percentages of cells were approximately 15.4% in early apoptosis and 43.7% in late apoptosis; after 36 h of incubation with colchicine at 20 μM, the corresponding values were 7.7% and 35.2%, respectively. Conclusions: Colchicine and luteolin from B. davidii have potential applications as adjuvant therapies for treating human carcinoma cells. These compounds could also induce apoptosis in tumor cells.

Houghton PJ, Mensah AY, Iessa N, Hong LY. Terpenoids in Buddleja: relevance to chemosystematics, chemical ecology and biological activity. Phytochemistry. 2003 Sep;64(2):385-93. doi: 10.1016/s0031-9422(03)00264-4. 

Abstract. The terpenoids reported from Buddleja species are described. The antifungal activity of chloroform extracts of B. cordata and B. davidii stembark against the soil fungi Fusarium culmorum and Sordari fimicola is reported, with buddledin A shown to be the major compound responsible. The terpenoids present support the view that the Buddlejaceae should be classified in a taxon with Scrophulariaceae rather than Loganiaceae. Ecological aspects of the terpenoids are considered in relation to insects and soil fungi and the role of terpenoids in the chemical basis of the use of Buddleja in traditional medicine is also discussed, especially with regard to their anti-inflammatory properties.

Zhang W, Tang SA, Duan HQ. Studies on chemical constituents of rhizome of Buddleia davidii Zhong Yao Cai. 2009 Apr;32(4):515-7. PMID: 19645234.

Abstract. Objective: To study the chemical constituents of Rhizome of Buddleia davidii. Methods: The chemical constituents were isolated by repeated column chromatography (Toyopearl HW-40C and HPLC) and their structures were elucidated on the basis of physico-chemical properties and spectroscopic features. Results: From the petroleum ether extract of the material, eight compounds were isolated. Their structures were identified as: Buddlindeterpene B(1), Buddledin B(2), Buddledin C(3), deacetyldihydrobuddledin A(4), dihydrobuddledin C(5), Suberosol B(6), Gadain (7) and Hinokinin (8). Conclusion: Compounds 1,4 - 8 are isolated from B. davidii for the first time.